Stephanie McCalla, Ph.D.

Assistant Professor

Contact Information

Phone: 406-994-2286
Office: 304 Cobleigh


B.S. in Bioengineering from University of California, San Diego (2005)
Post-Baccalaureate Researcher, Los Alamos National Laboratory (2005-2006)
PhD in Biomedical Engineering from Brown University (2011)
Postdoctoral Scholar in Chemistry and Chemical Engineering, California Institute of Technology (2012-2014)


EBIO 461: Principles of Biomedical Engineering (Spring 2016)
EBIO 491: Introduction to Biomedical Engineering (Spring 2015)
EBIO 324: Bioengineering Transport (Fall 2015)

Research Interests

Microfluidics, disease diagnostics, RNA/DNA/protein separation, detection, and quantification, mass transport in living systems


  1. Schlappi, T.*, McCalla, S.*, Schoepp, N., Ismagilov, R., “Flow-through capture and in situ amplification can enable rapid detection of a few single molecules of nucleic acids from several milliliters of solution” Analytical Chemistry, 2016, 88 (15): 7647–7653
  2. Achilli TM., McCalla S., Meyer J., Tripathi A., Morgan JR.  “Multilayer Spheroids To Quantify Drug Uptake and Diffusion in 3D”  Molecular Pharmaceutics, 2014, 11(7): 2071–2081
  3. Sun, B., Shen, F., McCalla, S., Kreutz, J., Karymov, M., Ismagilov, R.  “Mechanistic evaluation of the pros and cons of digital RT-LAMP for HIV-1 viral load quantification on a microfluidic device and improved efficiency via a two-step digital protocol”  Analytical Chemistry, 2013, 85(3): 1540-1546
  4. McCalla, S., Ong, C., Sarma, A., Opal, S., Artenstein, A., and Tripathi, A., “A Simple Method for Amplifying RNA targets (SMART)” Journal of Molecular Diagnostics, 2012, 14(4): 328-335, featured in the Nature Medicine blog (Spoonful of Medicine)
  5. Achilli, TM., McCalla, S., Tripathi, A., Morgan, J., “Quantification of the Kinetics and Extent of Self-Sorting in Three Dimensional Spheroids” Tissue Engineering Part C: Methods, Dec. 2011, 18(4): 302-309
  6. McCalla, S. and Tripathi, A. “Microfluidic Reactors for Diagnostic Applications” Annual Reviews of Biomedical Engineering, 2011, 13: 321-343
  7. McCalla, S. and Tripathi, A. “Quantifying transcription of clinically relevant immobilized DNA within a microfluidic reactor" Langmuir, 2010, 26(17): 14372–14379.
  8. McCalla, S., Luryi, A., and Tripathi, A. “Steric effects and mass transfer limitations surrounding amplification reactions on immobilized long and clinically relevant DNA templates" Langmuir, 2009, 25(11): 6168-6175.
  9. Goff, G., Brodnax, L., Cisneros, M., Peper, S., Field, S., Scott, B., Runde, W. “First Identification and Thermodynamic Characterization of the Ternary U(VI) Species, UO2(O2)(CO3)24−, in UO2−H2O2−K2CO3 Solutions” Inorganic Chemistry, 2008, 47(6) : 1984–1990
  10. Pepper, S.; Brodnax, L.; Field, S.; Zehnder,R.A.; Valdez,S.N.; Runde,WH. “Kinetic study of the oxidation dissolution of UO2 in aqueous carbonate media” Industrial Engineering and Chemical Research. 2004, 43: 8188-8193


  1. Ismagilov, R.F., McCalla, S.E., Schlappi, T., Huynh, T., Rolando, J., Liu, W., Ge, S., and Kreutz, J.E.,  Digital assay for quantifying and concentrating analytes.  Application PCT/US2014/047092.
  2. Ismagilov, R.F., Shen, F., Li, L., Hsu, Y.H., Begolo, S., Karymov, M., Selck, D., McCalla, S. and Homewood, P.J., Fluidic devices and systems for sample preparation or autonomous analysis. U.S. Patent Application 13/868,028.
  3. Morgan, J.R., Achilli, T.M., Tripathi, A. and McCalla, S., Differential effects of drugs on transport in a multi-layer 3d spheroid model. U.S. Patent Application 13/623,599.
  4. Tripathi, A., McCalla, S.E. and Lee, J., Magnetic bead separation apparatus and method. U.S. Patent Application 13/731,338.

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